polyclonal rabbit anti-gap-43 antibodies  (Millipore)

Journal: PLoS ONE

Article Title: A Peptide Mimetic Targeting Trans -Homophilic NCAM Binding Sites Promotes Spatial Learning and Neural Plasticity in the Hippocampus

doi: 10.1371/journal.pone.0023433

Figure Lengend Snippet: (a) 125,000 cells/cm 2 CGNs were left to differentiate for six days in the presence of high potassium (40 mM), followed by a 48 h incubation period with or without 15 nM EndoN. Cultures were double immunostained for GAP-43 (green) and PSA-NCAM (red). 12,500 cells/cm 2 Hippocampal neurons (b) and CGNs (c) were grown for 24 h in the presence or absence of 1.74 µM plannexin and treated with 30 nM EndoN ( b ) or 15 nM EndoN ( c ). Results from 4–5 experiments are expressed as a percentage ± SEM, with unstimulated controls set at 100%. *** p <0.001 vs. untreated controls; +++ p <0.001 vs. peptide-treated cultures without EndoN treatment.

Article Snippet: Polyclonal rabbit anti-GAP-43 antibodies were obtained from Chemicon (Temecula, CA, USA).

Techniques: Incubation

Journal: Neural Regeneration Research

Article Title: Olfactory ensheathing cell transplantation alters the expression of chondroitin sulfate proteoglycans and promotes axonal regeneration after spinal cord injury

doi: 10.4103/1673-5374.301023

Figure Lengend Snippet: Effects of OEC transplantation on GAP-43 immunopositivity in the injured spinal cord of rats. (A) GAP-43 immunopositivity in the injured area was detected using immunohistochemistry (original magnification 200×; scale bar: 200 µm). Arrows indicate GAP-43-positive cells. (B) Quantitative analysis of GAP-43 immunopositivity. Compared with the SCI group, GAP-43 immunopositivity in the OEC transplantation group was significantly higher at 2 and 4 weeks after SCI. Data are expressed as the mean ± SD ( n = 6). * P < 0.05 (unpaired t -test). GAP-43: Growth-associated protein 43; OEC: olfactory ensheathing cell; SCI, spinal cord injury.

Article Snippet: Rabbit anti-GAP-43 antibody (1:100) was purchased from Bioss (Cat# bs-0154R; Beijing, China), rabbit anti-NG2 antibody (1:200) was purchased from Abcam (Cat# ab83178; Cambridge, MA, USA), rabbit anti-neurocan antibody (1:200) was purchased from Millipore (Cat# ABT1382; Billerica, MA, USA), and rabbit anti-GFAP (1:100 Cat# BA0056) and -NF (1:100; Cat# A00279) antibodies were purchased from Boster (Wuhan, China).

Techniques: Transplantation Assay, Immunohistochemistry